Объем | 100 мкл |
Синонимы | ATP synthase subunit delta, mitochondrial (F-ATPase delta subunit), ATP5D |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | P30049 |
Иммуноген | Recombinant Human ATP synthase subunit delta, mitochondrial protein (1-168AA) |
Источник | Rabbit |
Видовая специфичность | Human, Mouse |
Применение | ELISA, WB, IHC, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200 |
Примечание | Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP turnover in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and of the central stalk which is part of the complex rotary element. Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | Antigen Affinity Purified |
Абревеатура | ATP synthase subunit delta, mitochondrial |
Области исследований | Cancer, Tags & Cell Markers, Metabolism, Signal transduction |
Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: ATP5F1D antibody at 1.94µg/ml
Lane 1: Raji whole cell lysate
Lane 2: NIH/3T3 whole cell lysate
Lane 3: A549 whole cell lysate
Lane 4: MDA-MB-231 whole cell lysate
Lane 5: HepG2 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 18 kDa
Observed band size: 18 kDa
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Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA002355ESR2HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA002355ESR2HU at dilution of 1:100
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