| Объем | 50 мкг |
| Синонимы | V-type proton ATPase subunit G 2 (V-ATPase subunit G 2) (V-ATPase 13 kDa subunit 2) (Vacuolar proton pump subunit G 2), ATP6V1G2, ATP6G ATP6G2 NG38 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | O95670 |
| Иммуноген | Recombinant Human V-type proton ATPase subunit G 2 protein (1-118AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
| Примечание | Catalytic subunit of the peripheral V1 complex of vacuolar ATPase (V-ATPase). V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Others |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: ATP6V1G2 antibody at 12µg/ml
Lane 1: Rat heart tissue
Lane 2: Mouse spleen tissue
Lane 3: Hela whole cell lysate
Lane 4: HepG2 whole cell lysate
Lane 5: A549 whole cell lysate
Lane 6: HT29 whole cell lysate
Lane 7: K562 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 14, 9 kDa
Observed band size: 14, 36 kDa
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Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA002409LA01HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA002409LA01HU at dilution of 1:100
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Immunofluorescence staining of A549 cells with CSB-PA002409LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Наименование: ATP6V1G2 Antibody.