Объем | 100 мкл |
Синонимы | ATP synthase subunit O, mitochondrial (Oligomycin sensitivity conferral protein) (OSCP), ATP5O, ATPO |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | P48047 |
Иммуноген | Recombinant Human ATP synthase subunit O, mitochondrial protein (24-213AA) |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, WB, IHC, Recommended dilution: WB:1:200-1:1000, IHC:1:20-1:200 |
Примечание | Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F0 domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha3beta3 subcomplex and subunit a/ATP6 static relative to the rotary elements. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | Antigen Affinity Purified |
Области исследований | Cancer, Tags & Cell Markers, Metabolism, Signal transduction |
Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: ATP5O antibody at 3µg/ml + HepG2 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 23 kDa
Observed band size: 23 kDa
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Immunohistochemistry of paraffin-embedded human skeletal muscle tissue using CSB-PA002379ESR2HU at dilution of 1:100
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