Объем | 100 мкл |
Синонимы | ATP synthase alpha chain, mitochondrial precursor, ATP5A, ATPA, EC 3.6.3.14 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | P25705 |
Иммуноген | Synthesized peptide derived from internal of human ATP5A1. |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, WB,WB:1:500-1:3000 |
Примечание | Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F1. Rotation of the central stalk against the surrounding alpha3beta3 subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites By similarity.<br/><br/>Kataoka H., Biochim. Biophys. Acta 1089:393-395(1991).<br/>Godbout R., Gene 123:195-201(1993).<br/>Akiyama S., Biochim. Biophys. Acta 1219:129-140(1994)<br/> |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Форма | liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Области исследований | Tags & Cell Markers,Metabolism,Signal transduction |
Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from RAW264.7 cells and 293 cells, using ATP5A1 antibody.
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