| Объем | 50 мкл |
| Синонимы | Ubiquitin carboxyl-terminal hydrolase BAP1 (EC 3.4.19.12) (BRCA1-associated protein 1) (Cerebral protein 6), BAP1, KIAA0272 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q92560 |
| Иммуноген | Recombinant Human Ubiquitin carboxyl-terminal hydrolase BAP1 protein (460-729AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, Recommended dilution: WB:1:200-1:1000 |
| Примечание | Deubiquitinating enzyme that plays a key role in chromatin by mediating deubiquitination of histone H2A and HCFC1. Catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1). Does not deubiquitinate monoubiquitinated histone H2B. Acts as a regulator of cell growth by mediating deubiquitination of HCFC1 N-terminal and C-terminal chains, with some specificity toward 'Lys-48'-linked polyubiquitin chains compared to 'Lys-63'-linked polyubiquitin chains. Deubiquitination of HCFC1 does not lead to increase stability of HCFC1. Interferes with the BRCA1 and BARD1 heterodimer activity by inhibiting their ability to mediate ubiquitination and autoubiquitination. It however does not mediate deubiquitination of BRCA1 and BARD1. Able to mediate autodeubiquitination via intramolecular interactions to couteract monoubiquitination at the nuclear localization signal (NLS), thereby protecting it from cytoplasmic sequestration (PubMed:24703950). Acts as a tumor suppressor. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Области исследований | Epigenetics and Nuclear Signaling, Cancer, Cell biology |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: BAP1 antibody at 8µg/ml + Hela whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 80 kDa
Observed band size: 80 kDa
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Наименование: BAP1 Antibody.