| Объем | 100 мкг |
| Синонимы | Ataxin-3 (EC 3.4.19.12) (Machado-Joseph disease protein 1) (Spinocerebellar ataxia type 3 protein), ATXN3, ATX3 MJD MJD1 SCA3 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P54252 |
| Иммуноген | Recombinant Human Ataxin-3 protein (1-288AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC, Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200 |
| Примечание | Deubiquitinating enzyme involved in protein homeostasis maintenance, transcription, cytoskeleton regulation, myogenesis and degradation of misfolded chaperone substrates. Binds long polyubiquitin chains and trims them, while it has weak or no activity against chains of 4 or less ubiquitins. Involved in degradation of misfolded chaperone substrates via its interaction with STUB1/CHIP: recruited to monoubiquitinated STUB1/CHIP, and restricts the length of ubiquitin chain attached to STUB1/CHIP substrates and preventing further chain extension. In response to misfolded substrate ubiquitination, mediates deubiquitination of monoubiquitinated STUB1/CHIP. Interacts with key regulators of transcription and represses transcription: acts as a histone-binding protein that regulates transcription. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Epigenetics and Nuclear Signaling, Neuroscience |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: ATXN3 antibody at 2µg/ml
Lane 1: 293T whole cell lysate
Lane 2: Mouse brain tissue
Lane 3: MCF-7 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 42, 36, 40, 21 kDa
Observed band size: 42 kDa
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Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA002443LA01HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human pancreatic tissue using CSB-PA002443LA01HU at dilution of 1:100
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Наименование: ATXN3 Antibody.