Объем | 100 мкл |
Синонимы | T-complex protein 1 subunit beta (TCP-1-beta) (CCT-beta), CCT2, 99D8.1 CCTB |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | P78371 |
Иммуноген | Recombinant Human T-complex protein 1 subunit beta protein (206-535AA) |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, WB, IHC, IP, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IP:1:200-1:2000 |
Примечание | Molecular chaperone, assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | Antigen Affinity Purified |
Абревеатура | T-complex protein 1 subunit beta |
Области исследований | Signal transduction |
Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: CCT2 antibody at 5.63µg/ml
Lane 1: Jurkat whole cell lysate
Lane 2: NIH/3T3 whole cell lysate
Lane 3: MCF-7 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 58, 53 kDa
Observed band size: 58 kDa
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Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA004856ESR1HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA004856ESR1HU at dilution of 1:100
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Immunoprecipitating CCT2 in Jurkat whole cell lysate
Lane 1: Rabbit monoclonal IgG (1µg) instead of CSB-PA004856ESR1HU in Jurkat whole cell lysate.
For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000)
Lane 2: CSB-PA004856ESR1HU (4µg) + Jurkat whole cell lysate (500µg)
Lane 3: Jurkat whole cell lysate (20µg)
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