| Объем | 50 мкг |
| Синонимы | Calcyclin-binding protein (CacyBP) (hCacyBP) (S100A6-binding protein) (Siah-interacting protein), CACYBP, S100A6BP SIP |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q9HB71 |
| Иммуноген | Recombinant Human Calcyclin-binding protein (2-228AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IF, IP, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000 |
| Примечание | May be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins. Probably serves as a molecular bridge in ubiquitin E3 complexes. Participates in the ubiquitin-mediated degradation of beta-catenin (CTNNB1). |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Calcyclin-binding protein |
| Области исследований | Neuroscience |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: 293 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, HUVEC whole cell lysate
All lanes: CACYBP antibody at 2.7µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 27, 9, 22 kDa
Observed band size: 27 kDa
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Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA872536LA01HU at dilution of 1:100
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Immunofluorescence staining of U251 cells with CSB-PA872536LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Immunoprecipitating CACYBP in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA872536LA01HU in Hela whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA872536LA01HU (8µg) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (10µg)
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Наименование: CACYBP Antibody.