Объем | 50 мкг |
Синонимы | M-phase inducer phosphatase 2 (EC 3.1.3.48) (Dual specificity phosphatase Cdc25B), CDC25B, CDC25HU2 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | P30305 |
Иммуноген | Recombinant Human M-phase inducer phosphatase 2 protein (1-195AA) |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
Примечание | Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Абревеатура | M-phase inducer phosphatase 2 |
Области исследований | Epigenetics and Nuclear Signaling, Cancer, Cell biology |
Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: Jurkat whole cell lysate, Raji whole cell lysate
All lanes: CDC25B antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 65, 64, 61, 68 kDa
Observed band size: 65 kDa
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Immunohistochemistry of paraffin-embedded human ovarian cancer using CSB-PA004995LA01HU at dilution of 1:100
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Immunofluorescent analysis of Hela cells using CSB-PA004995LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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