CEACAM1 AntibodyСпецификацияОбъем | 50 мкг | Синонимы | Carcinoembryonic antigen-related cell adhesion molecule 1 (Biliary glycoprotein 1) (BGP-1) (CD antigen CD66a), CEACAM1, BGP BGP1 | Клональность | Polyclonal Antibody | Организм | Human | uniprot | P13688 | Иммуноген | Recombinant Human Carcinoembryonic antigen-related cell adhesion molecule 1 protein (35-428AA) | Источник | Rabbit | Видовая специфичность | Human | Применение | ELISA, WB, IF, Recommended dilution: WB:1:1000-1:5000, IF:1:200-1:500 | Примечание | extracellular exosome, integral component of membrane, integral component of plasma membrane, membrane, plasma membrane, protein homodimerization activity, angiogenesis, blood coagulation, cell migration, homophilic cell adhesion via plasma membrane adhesion molecules | Клональность1 | Polyclonal | Изотип | IgG | Коньюгат | Non-conjugated | Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 | Форма | Liquid | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | Метод очистки | >95%, Protein G purified | Области исследований | Cancer, Tags & Cell Markers, Immunology, Microbiology | Ссылка на страницу на сайте производителя | ссылка | Western blot
All lanes: CEACAM1 antibody at 10µg/ml
Lane 1: HT29 whole cell lysate
Lane 2: HCT116 whole cell lysate
Lane 3: COLO205 whole cell lysate
Lane 4: HepG2 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 58, 46, 36, 39, 51, 47, 28, 44, 52 kDa
Observed band size: 58 kDa
| Immunofluorescence staining of HepG2 cells with CSB-PA10749A0Rb at 1:333, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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