| Объем | 50 мкг |
| Синонимы | Cation channel sperm-associated protein 2 (CatSper2), CATSPER2 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q96P56 |
| Иммуноген | Recombinant Human Cation channel sperm-associated protein 2 protein (5-112AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200, IF:1:50-1:200 |
| Примечание | Voltage-gated calcium channel that plays a central role in calcium-dependent physiological responses essential for successful fertilization, such as sperm hyperactivation, acrosome reaction and chemotaxis towards the oocyte. Activated by extracellular progesterone and prostaglandins following the sequence: progesterone > PGF1-alpha = PGE1 > PGA1 > PGE2 >> PGD2. The primary effect of progesterone activation is to shift voltage dependence towards more physiological, negative membrane potentials, it is not mediated by metabotropic receptors and second messengers. Sperm capacitation enhances the effect of progesterone by providing additional negative shift. Also activated by the elevation of intracellular pH. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Others |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: CATSPER2 antibody at 4µg/ml
Lane 1: Jurkat whole cell lysate
Lane 2: HepG2 whole cell lysate
Lane 3: 293T whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 63, 62, 49, 24 kDa
Observed band size: 63 kDa
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Immunohistochemistry of paraffin-embedded human spleen tissue using CSB-PA842731LA01HU at dilution of 1:100
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Immunofluorescent analysis of LO2 cells using CSB-PA842731LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Наименование: CATSPER2 Antibody.