| Объем | 50 мкл |
| Синонимы | Caspase recruitment domain-containing protein 9 (hCARD9), CARD9 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q9H257 |
| Иммуноген | Recombinant Human Caspase recruitment domain-containing protein 9 protein (237-536AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200 |
| Примечание | Adapter protein that plays a key role in innate immune response to a number of intracellular pathogens, such as C.albicans and L.monocytogenes. Is at the crossroads of ITAM-tyrosine kinase and the Toll-like receptors (TLR) and NOD2 signaling pathways. Probably controls various innate immune response pathways depending on the intracellular pathogen. In response to L.monocytogenes infection, acts by connecting NOD2 recognition of peptidoglycan to downstream activation of MAP kinases (MAPK) without activating NF-kappa-B. Also involved in activation of myeloid cells via classical ITAM-associated receptors and TLR: required for TLR-mediated activation of MAPK, while it is not required for TLR-induced activation of NF-kappa-B (By similarity). Controls CLEC7A (dectin-1)-mediated myeloid cell activation induced by the yeast cell wall component zymosan, leading to cytokine production and innate anti-fungal immunity: acts by regulating BCL10-MALT1-mediated NF-kappa-B activation pathway. Activates NF-kappa-B via BCL10. In response to the hyphal form of C.albicans, mediates CLEC6A (dectin-2)-induced I-kappa-B kinase ubiquitination, leading to NF-kappa-B activation via interaction with BCL10. In response to fungal infection, may be required for the development and subsequent differentiation of interleukin 17-producing T helper (TH-17) cells. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Области исследований | Cell biology |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: CARD9 antibody at 15µg/ml
Lane 1: THP-1 whole cell lysate
Lane 2: Jurkat whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 63, 57, 43 kDa
Observed band size: 63 kDa
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Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CSB-PA867133ESR1HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human spleen tissue using CSB-PA867133ESR1HU at dilution of 1:100
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Наименование: CARD9 Antibody.