Объем | 100 мкл |
Синонимы | cyclin D2, G1/S-specific cyclin D2, |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | P30279 |
Иммуноген | Synthesized non-phosphopeptide derived from human Cyclin D2 around the phosphorylation site of threonine 280 (A-S-T(p)-P-T). |
Источник | Rabbit |
Видовая специфичность | Human, Mouse |
Применение | ELISA, WB,WB:1:500-1:3000 |
Примечание | Regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D2/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex By similarity.<br/><br/>Xiong Y., Genomics 13:575-584(1992).<br/>Palmero I., Oncogene 8:1049-1054(1993).<br/>Kalnine N., Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.<br/> |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Форма | liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Области исследований | Epigenetics and Nuclear Signaling,Cancer,Cell biology |
Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from 293 cells, using Cyclin D2 (Ab-280) antibody.
|
| |