ООО «Лабораторная Диагностика»
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/ Каталог / Реагенты для научных исследований / Антитела / Поликлональные антитела Cusabio

CCND2 Antibody

Спецификация

Объем100 мкл
СинонимыG1/S-specific cyclin-D2, CCND2
КлональностьPolyclonal Antibody
ОрганизмHuman
uniprotP30279
ИммуногенRecombinant Human G1/S-specific cyclin-D2 protein (1-289AA)
ИсточникRabbit
Видовая специфичностьHuman
ПрименениеELISA, WB, IHC,IF, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:500, IF:1:50-1:200
ПримечаниеRegulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D2/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
Клональность1Polyclonal
ИзотипIgG
КоньюгатNon-conjugated
БуфферPBS with 0.02% sodium azide, 50% glycerol, pH7.3.
ФормаLiquid
ХранениеUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Метод очисткиAntigen Affinity purified
Области исследованийEpigenetics and Nuclear Signaling, Cancer, Cell biology
Ссылка на страницу на сайте производителяссылка
Immunohistochemistry of paraffin-embedded human cervical cancer using CSB-PA004812ESR2HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA004812ESR2HU at dilution of 1:100
Western Blot
Positive WB detected in: NIH/3T3 whole cell lysate
All lanes: CCND2 antibody at 2.25µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDa

IHC image of CSB-PA004812ESR2HU diluted at 1:225 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA004812ESR2HU diluted at 1:225 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of HepG2 cells with CSB-PA004812ESR2HU at 1:75, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  

Информация для заказа

Область использования:Производство:Cusabio
Метод:Антитела 
Объем:100 мкл 
Кат. номер:CSB-PA004812ESR2HU
Цена (с НДС 20%):по запросуВ корзину
CCND2 AntibodyНаименование: CCND2 Antibody.
Примечание: дополнительная информация (на английском языке).
   
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info@LD.ru   тел.: +7 495 369-20-43