Объем | 100 мкг |
Синонимы | Ciliary neurotrophic factor receptor subunit alpha (CNTF receptor subunit alpha) (CNTFR-alpha), CNTFR |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | P26992 |
Иммуноген | Recombinant Human Ciliary neurotrophic factor receptor subunit alpha protein (106-342AA) |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:200-1:1000, IHC:1:20-1:200, IF:1:50-1:200 |
Примечание | Binds to CNTF. The alpha subunit provides the receptor specificity. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Области исследований | Neuroscience, Signal transduction |
Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: CNTFR antibody at 2µg/ml + Hela whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa
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Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA005684EA01HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human skeletal muscle tissue using CSB-PA005684EA01HU at dilution of 1:100
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Immunofluorescence staining of SH-SY5Y cells with CSB-PA005684EA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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