CCR9 AntibodyСпецификация| Объем | 50 мкл | | Синонимы | C-C chemokine receptor type 9, C-C CKR-9, CC-CKR-9, CCR-9, G-protein coupled receptor 28, GPR-9-6, CDw199, CCR9, GPR28 | | Тип антител | Recombinant Antibody | | Species | Human | | UniProt ID | P51686 | | Иммуноген | A synthesized peptide derived from human CCR9 | | Видовая специфичность | Human, Mouse | | Применение | ELISA, WB, IF, IP, Recommended dilution: WB:1:500-1:5000, IF:1:20-1:200, IP:1:200-1:1000 | | Клональность | Monoclonal | | Изотип | Rabbit IgG | | Коньюгат | Non-conjugated | | Буффер | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | Liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Affinity-chromatography | | Области исследований | Immunology | | Аббревиатура | C-C chemokine receptor type 9 | | Примечание | Receptor for chemokine SCYA25/TECK. Subsequently transduces a signal by increasing the intracellular calcium ions level. | | Ссылка на страницу на сайте производителя | ссылка | Western Blot
Positive WB detected in: SH-SY5Y whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, HL-60 whole cell lysate, Hela whole cell lysate, U87 whole cell lysate, Mouse brain tissue
All lanes: CCR9 antibody at 1µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 43, 41 KDa
Observed band size: 43 KDa
 | Immunofluorescence staining of HepG2 cells with CSB-RA004848A0HU at 1:33, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
 | Immunoprecipitating CCR9 in HL-60 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-RA004848A0HU in HL-60 whole cell lysate.
For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-RA004848A0HU (3µg) + HL-60 whole cell lysate (500µg)
Lane 3: HL-60 whole cell lysate (20µg)
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Информация для заказа |
Наименование: CCR9 Antibody.