| Объем | 50 мкл |
| Синонимы | Histone H2B type 1-C/E/F/G/I (Histone H2B.1 A) (Histone H2B.a) (H2B/a) (Histone H2B.g) (H2B/g) (Histone H2B.h) (H2B/h) (Histone H2B.k) (H2B/k) (Histone H2B.l) (H2B/l), HIST1H2BC, HIST1H2BE, HIST1H2BF, HIST1H2BG, HIST1H2BI, H2BFL, H2BFH, H2BFG, H2BFA, H2BFK |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P62807 |
| Иммуноген | Peptide sequence around site of crotonyl-Lys(12) derived from Human Histone H2B |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IF, IP, ChIP, Recommended dilution: WB:1:200-1:2000, IF:1:50-1:200, IP:1:200-1:2000 |
| Примечание | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Абревеатура | Histone H2B type 1-C/E/F/G/I |
| Области исследований | Epigenetics and Nuclear Signaling |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, 293 whole cell lysate (all treated with 30mM sodium crotonylate for 4h)
All lanes: HIST1H2BC antibody at 0.97µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDa
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Immunofluorescence staining of Hela cells (treated with 30mM sodium crotonylate for 4h) with CSB-PA010403OA12crHU at 1:5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Immunoprecipitating HIST1H2BC in 293 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA010403OA12crHU in 293 whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA010403OA12crHU (5µg) + 293 whole cell lysate (500µg)
Lane 3: 293 whole cell lysate (20µg)
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Chromatin Immunoprecipitation Hela (106, treated with 30mM sodium crotonylate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H2BC (CSB-PA010403OA12crHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the ?-Globin promoter.
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Наименование: Crotonyl-HIST1H2BC (K12) Antibody.