Объем | 50 мкл |
Синонимы | cyclin-dependent kinase inhibitor 1A (p21, Cip1) |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | P38936 |
Иммуноген | Fusion protein of human CDKN1A |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, WB, IHC,ELISA:1:1000-1:5000, WB:1:200-1:1000, IHC:1:25-1:100 |
Примечание | This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Multiple alternatively spliced variants have been found for this gene. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | Antigen affinity purification |
Области исследований | Epigenetics and Nuclear Signaling,Cancer,Cell biology |
Ссылка на страницу на сайте производителя | ссылка |
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using CSB-PA297162(CDKN1A Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ?200)
|
Gel: 10+12%SDS-PAGE, Lysate: 40 ?g, Lane: Human liver cancer tissue, Primary antibody: CSB-PA297162(CDKN1A Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds
|
| |