CEBPA AntibodyСпецификация| Объем | 100 мкг | | Синонимы | CCAAT/enhancer-binding protein alpha (C/EBP alpha), CEBPA, CEBP | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | P49715 | | Иммуноген | Recombinant Human CCAAT/enhancer-binding protein alpha protein (1-124AA) | | Источник | Rabbit | | Видовая специфичность | Human | | Применение | ELISA, WB, IHC, Recommended dilution: WB:1:200-1:3000, IHC:1:20-1:200 | | Примечание | Transcription factor that coordinates proliferation arrest and the differentiation of myeloid progenitors, adipocytes, hepatocytes, and cells of the lung and the placenta. Binds directly to the consensus DNA sequence 5'-T[TG]NNGNAA[TG]-3' acting as an activator on distinct target genes (PubMed:11242107). During early embryogenesis, plays essential and redundant functions with CEBPB. Essential for the transition from common myeloid progenitors (CMP) to granulocyte/monocyte progenitors (GMP). Critical for the proper development of the liver and the lung (By similarity). Necessary for terminal adipocyte differentiation, is required for postnatal maintenance of systemic energy homeostasis and lipid storage (By similarity). To regulate these different processes at the proper moment and tissue, interplays with other transcription factors and modulators. Downregulates the expression of genes that maintain cells in an undifferentiated and proliferative state through E2F1 repression, which is critical for its ability to induce adipocyte and granulocyte terminal differentiation. Reciprocally E2F1 blocks adipocyte differentiation by binding to specific promoters and repressing CEBPA binding to its target gene promoters. Proliferation arrest also depends on a functional binding to SWI/SNF complex (PubMed:14660596). In liver, regulates gluconeogenesis and lipogenesis through different mechanisms. To regulate gluconeogenesis, functionally cooperates with FOXO1 binding to IRE-controlled promoters and regulating the expression of target genes such as PCK1 or G6PC. To modulate lipogenesis, interacts and transcriptionally synergizes with SREBF1 in promoter activation of specific lipogenic target genes such as ACAS2. In adipose tissue, seems to act as FOXO1 coactivator accessing to ADIPOQ promoter through FOXO1 binding sites (By similarity). | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 | | Форма | Liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | >95%, Protein G purified | | Абревеатура | CCAAT/enhancer-binding protein alpha | | Области исследований | Epigenetics and Nuclear Signaling, Cancer, Cardiovascular, Metabolism, Signal transduction | | Ссылка на страницу на сайте производителя | ссылка | Immunohistochemistry of paraffin-embedded human pancreatic tissue using CSB-PA005180HA01HU at dilution of 1:100
 | Western Blot
Positive WB detected in: A549 whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate
All lanes: CEBPA antibody at 2µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 38, 36, 26, 42 kDa
Observed band size: 38 kDa
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Наименование: CEBPA Antibody.