| Объем | 50 мкл |
| Синонимы | Centromere protein K (CENP-K) (Interphase centromere complex protein 37) (Protein AF-5alpha) (p33), CENPK, ICEN37 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q9BS16 |
| Иммуноген | Recombinant Human Centromere protein K protein (1-269AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IP, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IP:1:200-1:2000 |
| Примечание | Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Acts in coordination with CASC5/KNL1 to recruit the NDC80 complex to the outer kinetochore. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Абревеатура | Centromere protein K |
| Области исследований | Epigenetics and Nuclear Signaling |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: CENPK antibody at 3.98µg/ml
Lane 1: PC-3 whole cell lysate
Lane 2: 293T whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 32 kDa
Observed band size: 32 kDa
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Immunohistochemistry of paraffin-embedded human skin tissue using CSB-PA005214ESR1HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA005214ESR1HU at dilution of 1:100
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Immunoprecipitating CENPK in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA005214ESR1HU in Hela whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA005214ESR1HU (8µg) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (10µg)
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Наименование: CENPK Antibody.