Объем | 50 мкг |
Синонимы | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 (Oligosaccharyl transferase subunit DAD1) (Defender against cell death 1) (DAD-1), DAD1 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | P61803 |
Иммуноген | Recombinant Human Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 protein (1-113AA) |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200, IF:1:50-1:200 |
Примечание | Component of the N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). Loss of the DAD1 protein triggers apoptosis. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Абревеатура | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 protein |
Области исследований | Cell biology |
Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: Dolichyl-diphosphooligosaccharide-protein glycosyltransferase subunit DAD1 antibody at 2µg/ml
Lane 1: EC109 whole cell lysate
Lane 2: 293T whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/15000 dilution
Predicted band size: 12 .4 kDa
Observed band size: 12.4 kDa
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Immunohistochemistry of paraffin-embedded human prostate tissue using CSB-PA03124A0Rb at dilution of 1:100
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Immunofluorescent analysis of HepG2 cells using CSB-PA03124A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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