| Объем | 50 мкг |
| Синонимы | E3 ubiquitin-protein ligase CHFR (EC 2.3.2.27) (Checkpoint with forkhead and RING finger domains protein) (RING finger protein 196) (RING-type E3 ubiquitin transferase CHFR), CHFR, RNF196 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q96EP1 |
| Иммуноген | Recombinant Human E3 ubiquitin-protein ligase CHFR protein (141-422AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200 |
| Примечание | E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | E3 ubiquitin-protein ligase CHFR |
| Области исследований | Epigenetics and Nuclear Signaling, Cancer, Cell biology |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: HepG2 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, Mouse heart tissue
All lanes: CHFR antibody at 3.2µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000000 dilution
Predicted band size: 74, 73, 70, 64 kDa
Observed band size: 74 kDa
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Immunohistochemistry of paraffin-embedded human endometrial cancer using CSB-PA839315LA01HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA839315LA01HU at dilution of 1:100
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Наименование: CHFR Antibody.