Объем | 50 мкг |
Синонимы | Adapter molecule crk (Proto-oncogene c-Crk) (p38), CRK |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | P46108 |
Иммуноген | Recombinant Human Adapter molecule crk protein (40-196AA) |
Источник | Rabbit |
Видовая специфичность | Human, Rat |
Применение | ELISA, WB, IF, Recommended dilution: WB:1:500-1:5000, IF:1:50-1:200 |
Примечание | Isoform Crk-II: Regulates cell adhesion, spreading and migration. Mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4. May regulate the EFNA5-EPHA3 signaling. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Абревеатура | Adapter molecule crk |
Области исследований | Cancer, Signal transduction |
Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: HepG2 whole cell lysate, A549 whole cell lysate, PC-3 whole cell lysate, Hela whole cell lysate, HEK293 whole cell lysate
All lanes: CRK antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 34, 23 kDa
Observed band size: 37 kDa
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Immunofluorescent analysis of A549 cells using CSB-PA005979LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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