Crotonyl-HIST1H4A (K12) Antibody

Спецификация

Объем	50 мкл
Синонимы	Histone H4, HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4A, HIST2H4B, HIST4H4, H4/A H4FA, H4/I H4FI, H4/G H4FG, H4/B H4FB, H4/J H4FJ, H4/C H4FC, H4/H H4FH, H4/M H4FM, H4/E H4FE, H4/D H4FD, H4/K H4FK, H4/N H4F2 H4FN HIST2H4, H4/O H4FO,
Клональность	Polyclonal Antibody
Организм	Human
uniprot	P62805
Иммуноген	Peptide sequence around site of crotonyl-Lys(12) derived from Human Histone H4
Источник	Rabbit
Видовая специфичность	Human, Mouse, Rat
Применение	ELISA, WB, IF, Recommended dilution: WB:1:200-1:2000, IF:1:50-1:200
Примечание	Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Клональность1	Polyclonal
Изотип	IgG
Коньюгат	Non-conjugated
Буффер	Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Форма	Liquid
Хранение	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Метод очистки	Antigen Affinity Purified
Абревеатура	Histone H4
Области исследований	Epigenetics and Nuclear Signaling
Ссылка на страницу на сайте производителя	ссылка
Western Blot Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate (treated by 30mM sodium crotonylate for 4h) All lanes: HIST1H4A antibody at 0.15µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 12 kDa Observed band size: 12 kDa
Immunofluorescence staining of Hela cells (treated with 30mM sodium crotonylate for 4h) with CSB-PA010429OA12crHU, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Информация для заказа

Область использования: Поликлональные антитела Cusabio	Производство:	Cusabio
	Метод:	Антитела
	Объем:	50 мкл
	Кат. номер:	CSB-PA010429OA12crHU
Цена (с НДС 20%):	по запросу	В корзину
Наименование: Crotonyl-HIST1H4A (K12) Antibody. Примечание: дополнительная информация (на английском языке).