| Объем | 100 мкл |
| Синонимы | COP9 signalosome complex subunit 3 (SGN3) (Signalosome subunit 3) (JAB1-containing signalosome subunit 3), COPS3, CSN3 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q9UNS2 |
| Иммуноген | Recombinant Human COP9 signalosome complex subunit 3 protein (194-423AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC, IP, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IP:1:200-1:2000 |
| Примечание | Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Абревеатура | COP9 signalosome complex subunit 3 |
| Области исследований | Neuroscience, Cell biology |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: COPS3 antibody at 3.41µg/ml
Lane 1: MCF-7 whole cell lysate
Lane 2: Hela whole cell lysate
Lane 3: 293T whole cell lysate
Lane 4: HT29 whole cell lysate
Lane 5: Mouse heart tissue
Lane 6: Mouse brain tissue
Lane 7: Mouse ovarian tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 48, 46 kDa
Observed band size: 46 kDa
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Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA892483ESR1HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human heart tissue using CSB-PA892483ESR1HU at dilution of 1:100
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Immunoprecipitating COPS3 in Hela whole cell lysate
Lane 1: Rabbit monoclonal IgG (1µg) instead of CSB-PA892483ESR1HU in Hela whole cell lysate.
For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)
Lane 2: CSB-PA892483ESR1HU (4µg) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (20µg)
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Наименование: COPS3 Antibody.