CSF1R (Ab-809) AntibodyСпецификация| Объем | 100 мкл | | Синонимы | CD115, CSF-1-R, CSF1R, CSFMR, EC 2.7.10.1 | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | P07333 | | Иммуноген | Synthesized non-phosphopeptide derived from human CSFR around the phosphorylation site of tyrosine 809 (S-N-Y(p)-I-V). | | Источник | Rabbit | | Видовая специфичность | Human, Mouse | | Применение | ELISA, WB, IHC, IF,WB:1:500-1:3000, IHC:1:50-1:100, IF:1:100-1:500 | | Примечание | Tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines in response to IL34 and CSF1, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone and tooth development. Required for normal male and female fertility, and for normal development of milk ducts and acinar structures in the mammary gland during pregnancy. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration, and promotes cancer cell invasion. Activates several signaling pathways in response to ligand binding. Phosphorylates PIK3R1, PLCG2, GRB2, SLA2 and CBL. Activation of PLCG2 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, that then lead to the activation of protein kinase C family members, especially PRKCD. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to activation of the AKT1 signaling pathway. Activated CSF1R also mediates activation of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1, and of the SRC family kinases SRC, FYN and YES1. Activated CSF1R transmits signals both via proteins that directly interact with phosphorylated tyrosine residues in its intracellular domain, or via adapter proteins, such as GRB2. Promotes activation of STAT family members STAT3, STAT5A and/or STAT5B. Promotes tyrosine phosphorylation of SHC1 and INPP5D/SHIP-1. Receptor signaling is down-regulated by protein phosphatases, such as INPP5D/SHIP-1, that dephosphorylate the receptor and its downstream effectors, and by rapid internalization of the activated receptor.<br/><br/>Hampe A., Oncogene Res. 4:9-17(1989).<br/>Coussens L., Nature 320:277-280(1986).<br/>Andre C., Genomics 39:216-226(1997)<br/> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. | | Области исследований | Cardiovascular,Immunology,Signal transduction | | Ссылка на страницу на сайте производителя | ссылка | Immunohistochemistry analysis of paraffin-embedded human brain tissue using CSFR (Ab-809) antibody.
 | Immunofluorescence analysis of HeLa cells, using CSFR (Ab-809) antibody.
 | Western blot analysis of extracts from HT-29 cells, using CSFR (Ab-809) antibody.
 | Western blot analysis of extracts from HeLa cells (Lane 2) and HepG2 cells (Lane 3), using CSFR (Ab-809) antiobdy. The lane on the left is treated with synthesized peptide.
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Наименование: CSF1R (Ab-809) Antibody.