Объем | 50 мкг |
Синонимы | Casein kinase I isoform epsilon (CKI-epsilon) (CKIe) (EC 2.7.11.1), CSNK1E |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | P49674 |
Иммуноген | Recombinant Human Casein kinase I isoform epsilon protein (17-231AA) |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, WB, IHC, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200 |
Примечание | Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. Can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates DVL1. Central component of the circadian clock. May act as a negative regulator of circadian rhythmicity by phosphorylating PER1 and PER2. Retains PER1 in the cytoplasm. Inhibits cytokine-induced granuloytic differentiation. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Области исследований | Signal transduction |
Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: SH-SY5Y whole cell lysate
All lanes: CSNK1E antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDa
![](http://www.cusabio.cn/images/antibody/5/CSB-PA01315A0Rb-WB-2.jpg) |
IHC image of CSB-PA01315A0Rb diluted at 1:150 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
![](http://www.cusabio.cn/images/antibody/5/CSB-PA01315A0Rb-IHC-1.jpg) |
IHC image of CSB-PA01315A0Rb diluted at 1:150 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
![](http://www.cusabio.cn/images/antibody/5/CSB-PA01315A0Rb-IHC-2.jpg) |
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