CUL2 AntibodyСпецификация| Объем | 100 мкл | | Синонимы | Cullin-2, CUL-2, | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | Q13617 | | Иммуноген | Synthesized peptide derived from human Cullin 2. | | Источник | Rabbit | | Видовая специфичность | Human, Mouse | | Применение | ELISA, WB, IHC, IF,WB:1:500-1:3000, IHC:1:50-1:100, IF:1:100-1:500 | | Примечание | Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 By similarity. The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF).<br/><br/>Seiji Shiraishi, J. Biol. Chem., Mar 2007, 282: 9017 - 9028.<br/>Isabelle Groulx, J. Biol. Chem., Mar 2000, 275: 8991.<br/>Dimitris Liakopoulos, PNAS, May 1999, 96: 5510.<br/>Kyungwon Huh, AACR Meeting Abstracts, Apr 2006, 2006: 1326.<br/> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. | | Области исследований | Cancer,Cell biology | | Ссылка на страницу на сайте производителя | ссылка | Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Cullin 2 antibody.
 | Immunofluorescence analysis of NIH/3T3 cells, using Cullin 2 antibody.
 | Western blot analysis of extracts from LOVO cells, using Cullin 2 antibody.
 | Western blot analysis of extracts from HepG2 cells (Lane 2), using Cullin 2 antiobdy. The lane on the left is treated with systhesized peptide.
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Наименование: CUL2 Antibody.