| Объем | 100 мкг |
| Синонимы | Extended synaptotagmin-2 (E-Syt2) (Chr2Syt), ESYT2, FAM62B KIAA1228 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | A0FGR8 |
| Иммуноген | Recombinant Human Extended synaptotagmin-2 protein (567-736AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, IHC, IF, Recommended dilution: IHC:1:500-1:1000, IF:1:200-1:500 |
| Примечание | Tethers the endoplasmic reticulum to the cell membrane and promotes the formation of appositions between the endoplasmic reticulum and the cell membrane. Binds glycerophospholipids in a barrel-like domain and may play a role in cellular lipid transport. Plays a role in FGF signaling via its role in the rapid internalization of FGFR1 that has been activated by FGF1 binding, this occurs most likely via the AP-2 complex. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Extended synaptotagmin-2 |
| Области исследований | Cell biology |
| Ссылка на страницу на сайте производителя | ссылка |
Immunofluorescence staining of A549 cells with CSB-PA008279LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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IHC image of CSB-PA008279LA01HU diluted at 1:600 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Наименование: ESYT2 Antibody.