| Объем | 50 мкл |
| Синонимы | Histone-lysine N-methyltransferase EZH1 (EC 2.1.1.43) (ENX-2) (Enhancer of zeste homolog 1), EZH1, KIAA0388 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q92800 |
| Иммуноген | Recombinant Human Histone-lysine N-methyltransferase EZH1 protein (160-280AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC, Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200 |
| Примечание | Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH2-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Области исследований | Epigenetics and Nuclear Signaling |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: Histone-lysine N-methyltransferase EZH1 antibody at 4µg/ml
Lane 1: Hela whole cell lysate
Lane 2: HL60 whole cell lysate
Lane 3: Mouse gonad tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 86, 87, 81, 77, 69 kDa
Observed band size: 86 kDa
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Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA852913DSR2HU at dilution of 1:100
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Наименование: EZH1 Antibody.