EZH2 AntibodyСпецификация| Объем | 100 мкл | | Синонимы | Histone-lysine N-methyltransferase EZH2 (EC 2.1.1.43) (ENX-1) (Enhancer of zeste homolog 2) (Lysine N-methyltransferase 6), EZH2, KMT6 | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | Q15910 | | Иммуноген | Recombinant Human Histone-lysine N-methyltransferase EZH2 protein (1-250AA) | | Источник | Rabbit | | Видовая специфичность | Human, Mouse | | Применение | ELISA, WB, Recommended dilution: WB:1:500-1:5000 | | Примечание | Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4 and the nuclear receptor RORA. Regulates the circadian clock via histone methylation at the promoter of the circadian genes. Essential for the CRY1/2-mediated repression of the transcriptional activation of PER1/2 by the CLOCK-ARNTL/BMAL1 heterodimer, involved in the di and trimethylation of 'Lys-27' of histone H3 on PER1/2 promoters which is necessary for the CRY1/2 proteins to inhibit transcription. | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. | | Форма | Liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Antigen Affinity Purified | | Области исследований | Epigenetics and Nuclear Signaling, Cancer | | Ссылка на страницу на сайте производителя | ссылка | Western blot
All lanes: Histone-lysine N-methyltransferase EZH2 antibody at 12µg/ml
Lane 1: A431 whole cell lysate
Lane 2: Jurkat whole cell lysate
Lane 3: Mouse kidney tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 86, 87, 82, 85, 80 kDa
Observed band size: 86 kDa
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Наименование: EZH2 Antibody.