Объем | 50 мкг |
Синонимы | D-amino-acid oxidase (DAAO) (DAMOX) (DAO) (EC 1.4.3.3), DAO, DAMOX |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | P14920 |
Иммуноген | Recombinant Human D-amino-acid oxidase protein (1-347AA) |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, WB, IHC, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:500 |
Примечание | Regulates the level of the neuromodulator D-serine in the brain. Has high activity towards D-DOPA and contributes to dopamine synthesis. Could act as a detoxifying agent which removes D-amino acids accumulated during aging. Acts on a variety of D-amino acids with a preference for those having small hydrophobic side chains followed by those bearing polar, aromatic, and basic groups. Does not act on acidic amino acids. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Области исследований | Tags & Cell Markers, Metabolism, Signal transduction |
Ссылка на страницу на сайте производителя | ссылка |
Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA006494LA01HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA006494LA01HU at dilution of 1:100
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Western Blot
Positive WB detected in: HepG2 whole cell lysate
All lanes: DAO antibody at 7.4µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDa
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IHC image of CSB-PA006494LA01HU diluted at 1:500 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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