Объем | 100 мкг |
Синонимы | Protein DBF4 homolog B (Activator of S phase kinase-like protein 1) (ASK-like protein 1) (Chiffon homolog B) (Dbf4-related factor 1), DBF4B, ASKL1 DRF1 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | Q8NFT6 |
Иммуноген | Recombinant Human Protein DBF4 homolog B protein (1-300AA) |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, WB, IHC, Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200 |
Примечание | Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S and M phases. The complex CDC7-DBF4B selectively phosphorylates MCM2 subunit at 'Ser-40' and then is involved in regulating the initiation of DNA replication during cell cycle. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Области исследований | Epigenetics and Nuclear Signaling, Cancer, Cell biology, Signal transduction |
Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: DBF4B antibody at 4µg/ml
Lane 1: MCF-7 whole cell lysate
Lane 2: A549 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 68, 48, 55, 19 kDa
Observed band size: 48 kDa
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Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA822784LA01HU at dilution of 1:100
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