| Объем | 100 мкл |
| Синонимы | Mitotic spindle-associated MMXD complex subunit MIP18 (MSS19-interacting protein of 18 kDa) (Protein FAM96B), FAM96B, MIP18 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q9Y3D0 |
| Иммуноген | Recombinant Human Mitotic spindle-associated MMXD complex subunit MIP18 protein (1-163AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC, IP, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IP:1:200-1:2000 |
| Примечание | Component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into extramitochondrial Fe/S proteins. As part of the mitotic spindle-associated MMXD complex it plays a role in chromosome segregation, probably by facilitating iron-sulfur cluster assembly into ERCC2/XPD. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Абревеатура | Mitotic spindle-associated MMXD complex subunit MIP18 |
| Области исследований | Cancer, Cell biology |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: FAM96B antibody at 7.23µg/ml
Lane 1: Mouse gonadal tissue
Lane 2: MCF-7 whole cell lysate
Lane 3: Hela whole cell lysate
Lane 4: K562 whole cell lysate
Lane 5: A375 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 18 kDa
Observed band size: 18 kDa
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Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA897544ESR1HU at dilution of 1:100
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Immunoprecipitating FAM96B in Hela whole cell lysate
Lane 1: Rabbit monoclonal IgG (1µg) instead of CSB-PA897544ESR1HU in Hela whole cell lysate.
For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)
Lane 2: CSB-PA897544ESR1HU (4µg) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (20µg)
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Наименование: FAM96B Antibody.