| Объем | 50 мкл |
| Синонимы | Probable ATP-dependent RNA helicase DDX5 (EC 3.6.4.13) (DEAD box protein 5) (RNA helicase p68), DDX5, G17P1 HELR HLR1 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P17844 |
| Иммуноген | Recombinant Human Probable ATP-dependent RNA helicase DDX5 protein (1-290AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200 |
| Примечание | Involved in the alternative regulation of pre-mRNA splicing, its RNA helicase activity is necessary for increasing tau exon 10 inclusion and occurs in a RBM4-dependent manner. Binds to the tau pre-mRNA in the stem-loop region downstream of exon 10. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. Involved in transcriptional regulation, the function is independent of the RNA helicase activity. Transcriptional coactivator for estrogen receptor ESR1 and androgen receptor AR. Increases ESR1 AF-1 domain-mediated transactivation and ESR1 AF-1 and AF-2 domains transcriptional synergistic activity. Synergizes with DDX17 and SRA1 RNA to activate MYOD1 transcriptional activity and involved in skeletal muscle differentiation. Transcriptional coactivator for p53/TP53 and involved in p53/TP53 transcriptional response to DNA damage and p53/TP53-dependent apoptosis. Transcriptional coactivator for RUNX2 and involved in regulation of osteoblast differentiation. Acts as transcriptional repressor in a promoter-specicic manner, the function probbaly involves association with histone deacetylases, such as HDAC1. As component of a large PER complex is involved in the inhibition of 3' transcriptional termination of circadian target genes such as PER1 and NR1D1 and the control of the circadian rhythms. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Области исследований | Epigenetics and Nuclear Signaling |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: Probable ATP-dependent RNA helicase DDX5 antibody at 3µg/ml
Lane 1: NIH/3T3 whole cell lysate
Lane 2: Hela whole cell lysate
Lane 3: 293T whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 70, 61 kDa
Observed band size: 70 kDa
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Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA006630ESR2HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA006630ESR2HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA006630ESR2HU at dilution of 1:100
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Наименование: DDX5 Antibody.