Объем | 50 мкг |
Синонимы | Apoptosis-enhancing nuclease (EC 3.1.-.-) (Interferon-stimulated 20 kDa exonuclease-like 1), AEN, ISG20L1 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | Q8WTP8 |
Иммуноген | Recombinant Human Apoptosis-enhancing nuclease protein (117-325AA) |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, IF, Recommended dilution: IF:1:200-600 |
Примечание | Exonuclease with activity against single- and double-stranded DNA and RNA. Mediates p53-induced apoptosis. When induced by p53 following DNA damage, digests double-stranded DNA to form single-stranded DNA and amplifies DNA damage signals, leading to enhancement of apoptosis. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Абревеатура | Apoptosis-enhancing nuclease |
Области исследований | Cancer, Cell biology |
Ссылка на страницу на сайте производителя | ссылка |
Immunofluorescence staining of HepG2 cells with CSB-PA819883LA01HU at 1:530, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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