Объем | 100 мкг |
Тип антител | Tag/Control Antibodies |
Иммуноген | Synthetic Peptide |
Источник | Mouse |
Иммуноген 1 | All |
Применение | ELISA,WB,IF,IP;Recommended dilution:WB:1:500-1:5000,IF:1:50-1:200,IP:1:200-1:2000 |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. |
Форма | liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Ссылка на страницу на сайте производителя | ссылка |
IP antibody use: 5ug Flag Mouse IgG1 per ml Lysate, WB 1:5000 1) untransfected 293 cell lysate 2) transfected 293 cell lysate with Flag-tag fusion protein 3) IP (transfected 293+ normal Mouse IgG+Protein G agarose) 4) IP (transfected 293+anti- Flag mA
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IF analysis of 293 cells transfected with a Flag-tag protein,1:2000 dilution (blue DAPI, red anti-Flag)
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1ug Flag fusion protein+ Primary antibody dilution at 1:10000
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