| Объем | 100 мкл |
| Синонимы | DNA fragmentation factor subunit alpha (DNA fragmentation factor 45 kDa subunit) (DFF-45) (Inhibitor of CAD) (ICAD), DFFA, DFF1 DFF45 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | O00273 |
| Иммуноген | Recombinant Human DNA fragmentation factor subunit alpha protein (1-331AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IP, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IP:1:200-1:2000 |
| Примечание | Inhibitor of the caspase-activated DNase (DFF40). |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Области исследований | Epigenetics and Nuclear Signaling, Cancer, Cell biology, Metabolism |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: DFFA antibody at 10µg/ml
Lane 1: Hela whole cell lysate
Lane 2: 293T whole cell lysate
Lane 3: PC-3 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 37, 30 kDa
Observed band size: 37 kDa
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Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA006737DSR2HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA006737DSR2HU at dilution of 1:100
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Immunoprecipitating DFFA in Hela whole cell lysate
Lane 1: Rabbit monoclonal IgG (1µg) instead of CSB-PA006737DSR2HU in Hela whole cell lysate.
For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)
Lane 2: CSB-PA006737DSR2HU (4µg) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (20µg)
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Наименование: DFFA Antibody.