| Объем | 50 мкл |
| Синонимы | Histone H1.2 (Histone H1c) (Histone H1d) (Histone H1s-1), HIST1H1C, H1F2 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P16403 |
| Иммуноген | Peptide sequence around site of Di-methyl-Lys (45) derived from Human Histone H1.2 |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, ICC, IP, ChIP, Recommended dilution: WB:1:1000-1:5000, ICC:1:10-1:100, IP:1:200-1:2000 |
| Примечание | Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity). |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Абревеатура | Histone H1.2 |
| Области исследований | Epigenetics and Nuclear Signaling |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: HepG2 whole cell lysate, K562 whole cell lysate, HL-60 whole cell lysate
All lanes: HIST1H1C antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa
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Immunocytochemistry analysis of CSB-PA010378OA45me2HU diluted at 1:10 and staining in Hela cells performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H1C (CSB-PA010378OA45me2HU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the ?-Globin promoter.
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Immunoprecipitating HIST1H1C in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA010378OA45me2HU in Hela whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA010378OA45me2HU (5µg) + Hela whole cell lysate (1mg)
Lane 3: Hela whole cell lysate (20µg)
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Наименование: Di-methyl-HIST1H1C (K45) Antibody.