Объем | 50 мкг |
Синонимы | Deoxyribonuclease-1 (EC 3.1.21.1) (Deoxyribonuclease I) (DNase I) (Dornase alfa), DNASE1, DNL1 DRNI |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | P24855 |
Иммуноген | Recombinant Human Deoxyribonuclease-1 protein (78-231AA) |
Источник | Rabbit |
Видовая специфичность | Human, Mouse |
Применение | ELISA, WB, IHC, Recommended dilution: WB:1:500-1:5000, IHC:1:500-1:1000 |
Примечание | Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization (By similarity). |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Абревеатура | Deoxyribonuclease-1 |
Области исследований | Epigenetics and Nuclear Signaling, Cancer, Cell biology, Metabolism |
Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: Mouse brain tissue
All lanes: DNASE1 antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 32, 20 kDa
Observed band size: 32 kDa
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IHC image of CSB-PA007049LA01HU diluted at 1:600 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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