| Объем | 50 мкг |
| Синонимы | Docking protein 5 (Downstream of tyrosine kinase 5) (Insulin receptor substrate 6) (IRS-6) (IRS6), DOK5, C20orf180 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q9P104 |
| Иммуноген | Recombinant Human Docking protein 5 protein (1-198AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
| Примечание | DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK5 functions in RET-mediated neurite outgrowth and plays a positive role in activation of the MAP kinase pathway. Putative link with downstream effectors of RET in neuronal differentiation. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: Docking protein 5 antibody at 2µg/ml + Mouse kidney tissue
Secondary
Goat polyclonal to rabbit IgG at 1/15000 dilution
Predicted band size: 36, 23 kDa
Observed band size: 36 kDa
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Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA007111LA01HU at dilution of 1:100
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Immunofluorescence staining of A549 cells with CSB-PA007111LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Наименование: DOK5 Antibody.