Объем | 50 мкл |
Синонимы | Mitochondrial import inner membrane translocase subunit TIM14 (DnaJ homolog subfamily C member 19), DNAJC19, TIM14 TIMM14 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | Q96DA6 |
Иммуноген | Recombinant Human Mitochondrial import inner membrane translocase subunit TIM14 protein (1-116AA) |
Источник | Rabbit |
Видовая специфичность | Human, Mouse |
Применение | ELISA, WB, IHC, IP, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IP:1:200-1:2000 |
Примечание | Probable component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. May act as a co-chaperone that stimulate the ATP-dependent activity (By similarity). |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | Antigen Affinity Purified |
Абревеатура | Mitochondrial import inner membrane translocase subunit TIM14 |
Области исследований | Neuroscience, Signal transduction |
Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: DNAJC19 antibody at 0.96µg/ml
Lane 1: Mouse kidney tissue
Lane 2: A549 whole cell lysate
Lane 3: HL60 whole cell lysate
Lane 4: Hela whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 13, 11 kDa
Observed band size: 13 kDa
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Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA853400ESR1HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA853400ESR1HU at dilution of 1:100
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Immunoprecipitating DNAJC19 in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA853400ESR1HU in Hela whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA853400ESR1HU (8µg) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (10µg)
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