| Объем | 100 мкл |
| Синонимы | DNA (cytosine-5)-methyltransferase 3B, EC 2.1.1.37, Dnmt3b, DNA methyltransferase HsaIIIB, DNA MTase HsaIIIB |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q9UBC3 |
| Иммуноген | Synthesized peptide derived from N-terminal of human DNMT3B. |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IF,WB:1:500-1:3000, IHC:1:50-1:100, IF:1:100-1:500 |
| Примечание | Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing By similarity. In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Isoforms 4 and 5 are probably not functional due to the deletion of two conserved methyltransferase motifs. Function as transcriptional corepressor by associating with ZHX1.<br/><br/>Deloukas P., Nature 414:865-871(2001).<br/>Li H., J. Biol. Chem. 281:19489-19500(2006).<br/>Wijmenga C., Hum. Mutat. 16:509-517(2000).<br/> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Области исследований | Epigenetics and Nuclear Signaling |
| Ссылка на страницу на сайте производителя | ссылка |
Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using DNMT3B antibody.
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Immunofluorescence analysis of HeLa cells, using DNMT3B antibody.
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Western blot analysis of extracts from HT-29 cells, using DNMT3B antibody.
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Western blot analysis of extracts from HuvEc cells (Lane 2), using DNMT3B antiobdy. The lane on the left is treated with synthesized peptide.
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Наименование: DNMT3B Antibody.