| Объем | 100 мкг |
| Синонимы | Glycine--tRNA ligase (EC 3.6.1.17) (EC 6.1.1.14) (Diadenosine tetraphosphate synthetase) (AP-4-A synthetase) (Glycyl-tRNA synthetase) (GlyRS), GARS |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P41250 |
| Иммуноген | Recombinant Human Glycine--tRNA ligase protein (1-389AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB, IHC, IF, IP, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000 |
| Примечание | Catalyzes the attachment of glycine to tRNA(Gly). Is also able produce diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Glycine--tRNA ligase |
| Области исследований | Epigenetics and Nuclear Signaling |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: U87 whole cell lysate, Mouse brain tissue, Mouse liver tissue
All lanes: GARS antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 84 kDa
Observed band size: 84 kDa
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Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA009262EA01HU at dilution of 1:100
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Immunofluorescent analysis of HepG2 cells using CSB-PA009262EA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Immunoprecipitating GARS in HL60 whole cell lysate
Lane 1: Rabbit monoclonal IgG (1µg) instead of CSB-PA009262EA01HU in HL60 whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA009262EA01HU (8µg) + HL60 whole cell lysate (500µg)
Lane 3: HL60 whole cell lysate (20µg)
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Наименование: GARS Antibody.