| Объем | 50 мкг |
| Синонимы | Protein ECT2 (Epithelial cell-transforming sequence 2 oncogene), ECT2 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q9H8V3 |
| Иммуноген | Recombinant Human Protein ECT2 protein (283-453AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IP, Recommended dilution: WB:1:500-1:5000, IHC:1:500-1:1000, IP:1:200-1:2000 |
| Примечание | Guanine nucleotide exchange factor (GEF) that catalyzes the exchange of GDP for GTP. Promotes guanine nucleotide exchange on the Rho family members of small GTPases, like RHOA, RHOC, RAC1 and CDC42. Required for signal transduction pathways involved in the regulation of cytokinesis. Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Regulates the translocation of RHOA from the central spindle to the equatorial region. Plays a role in the control of mitotic spindle assembly, regulates the activation of CDC42 in metaphase for the process of spindle fibers attachment to kinetochores before chromosome congression. Involved in the regulation of epithelial cell polarity, participates in the formation of epithelial tight junctions in a polarity complex PARD3-PARD6-protein kinase PRKCQ-dependent manner. Plays a role in the regulation of neurite outgrowth. Inhibits phenobarbital (PB)-induced NR1I3 nuclear translocation. Stimulates the activity of RAC1 through its association with the oncogenic PARD6A-PRKCI complex in cancer cells, thereby acting to coordinately drive tumor cell proliferation and invasion. Also stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Protein ECT2 |
| Области исследований | Others |
| Ссылка на страницу на сайте производителя | ссылка |
IHC image of CSB-PA888001LA01HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunoprecipitating ECT2 in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA888001LA01HU in Hela whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/50000)
Lane 2: CSB-PA888001LA01HU (6µg) + Hela whole cell lysate (1mg)
Lane 3: Hela whole cell lysate (20µg)
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Western Blot
Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, 293T whole cell lysate
All lanes:ECT2 antibody at 2.7µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 104, 100, 109, 101 kDa
Observed band size: 104 kDa
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Наименование: ECT2 Antibody.