| Объем | 50 мкг |
| Синонимы | Transcription factor E2F1 (E2F-1) (PBR3) (Retinoblastoma-associated protein 1) (RBAP-1) (Retinoblastoma-binding protein 3) (RBBP-3) (pRB-binding protein E2F-1), E2F1, RBBP3 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q01094 |
| Иммуноген | Recombinant Human Transcription factor E2F1 protein (133-362AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IP, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IP:1:200-1:2000 |
| Примечание | Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters (PubMed:20176812). |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Transcription factor E2F1 |
| Области исследований | Epigenetics and Nuclear Signaling, Cancer |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: E2F1 antibody at 7 µg/ml + 3T3 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa
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Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA007340LA01HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human melanoma using CSB-PA007340LA01HU at dilution of 1:100
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Immunoprecipitating E2F1 in 3T3 whole cell lysate
Lane 1: Rabbit control IgG (1µg) instead of CSB-PA007340LA01HU in 3T3 whole cell lysate.
For western blotting, a HRP-conjugated light chain specific antibody was used as the secondary antibody (1/50000)
Lane 2: CSB-PA007340LA01HU (8µg) + 3T3 whole cell lysate (500µg)
Lane 3: 3T3 whole cell lysate (10µg)
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Наименование: E2F1 Antibody.