Объем | 50 мкг |
Синонимы | Extracellular matrix protein 1 (Secretory component p85), ECM1 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | Q16610 |
Иммуноген | Recombinant Human Extracellular matrix protein 1 protein (386-540AA) |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, WB, IHC, Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500 |
Примечание | Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Абревеатура | Extracellular matrix protein 1 |
Области исследований | Tags & Cell Markers, Immunology |
Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: MCF-7 whole cell lysate
All lanes: ECM1 antibody at 4.5µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 61, 47, 20, 64 kDa
Observed band size: 47 kDa
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IHC image of CSB-PA007383LA01HU diluted at 1:200 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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