Объем | 50 мкг |
Синонимы | ARF GTPase-activating protein GIT1 (ARF GAP GIT1) (Cool-associated and tyrosine-phosphorylated protein 1) (CAT-1) (CAT1) (G protein-coupled receptor kinase-interactor 1) (GRK-interacting protein 1), GIT1 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | Q9Y2X7 |
Иммуноген | Recombinant Human ARF GTPase-activating protein GIT1 protein (470-642AA) |
Источник | Rabbit |
Видовая специфичность | Human, Mouse |
Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
Примечание | GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine-phosphorylated paxillin in cytoplasmic complexes. Involved in the regulation of cytokinesis, the function may involve SDCCAG3 and PTPN13 (By similarity). |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Абревеатура | ARF GTPase-activating protein GIT1 |
Области исследований | Signal transduction |
Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: Mouse brain tissue
All lanes: GIT1 antibody at 3.5µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 85, 20, 86 kDa
Observed band size: 85 kDa
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Immunohistochemistry of paraffin-embedded human thyroid tissue using CSB-PA009439LA01HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human pancreatic tissue using CSB-PA009439LA01HU at dilution of 1:100
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Immunofluorescent analysis of Hela cells using CSB-PA009439LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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