| Объем | 100 мкл |
| Синонимы | EC 2.7.10.1, epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian), Epidermal growth factor receptor precursor, ERBB1 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P00533 |
| Иммуноген | Synthesized peptide derived from Internal of human EGFR. |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB,WB:1:500-1:3000 |
| Примечание | Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin. Isoform 2 may act as an antagonist of EGF action.<br/><br/>Ullrich A., Nature 309:418-425(1984).<br/>Ilekis J.V., Mol. Reprod. Dev. 41:149-156(1995).<br/>Reiter J.L. Nucleic Acids Res. 24:4050-4056(1996)<br/> |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Области исследований | Cancer,Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot analysis of extracts from JK cells (Lane 2) and HuvEc cells (Lane 3), using EGFR (Ab-1071) antiobdy. The lane on the left is treated with synthesized peptide.
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Наименование: EGFR (Ab-1071) Antibody.