| Объем | 50 мкг |
| Синонимы | Eukaryotic translation initiation factor 3 subunit I (eIF3i) (Eukaryotic translation initiation factor 3 subunit 2) (TGF-beta receptor-interacting protein 1) (TRIP-1) (eIF-3-beta) (eIF3 p36), EIF3I, EIF3S2 TRIP1 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q13347 |
| Иммуноген | Recombinant Human Eukaryotic translation initiation factor 3 subunit I protein (1-323AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Rat |
| Применение | ELISA, WB, IF, Recommended dilution: WB:1:2000-1:10000, IF:1:50-1:500 |
| Примечание | EIF3S2 is the largest of the EIFs. It consists of at least 10 nonidentical subunits in mammals. In S. cerevisiae the p39 subunit contains WD repeats, these are thought to mediate protein-protein interactions. The p39 protein appears to be essential for maintaining the integrity of the yeast EIF3 complex. The mammalian EIF3-p36 subunit is homologous to yeast p39. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Eukaryotic translation initiation factor 3 subunit I protein |
| Области исследований | Epigenetics and Nuclear Signaling |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: Rat brain tissue
All lanes: EIF3I antibody at 3.2µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 37 kDa
Observed band size: 37 kDa
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Immunofluorescence staining of Hela cells with CSB-PA03105A0Rb at 1:266, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Наименование: EIF3I Antibody.